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KMID : 0043319950180010012
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Archives of Pharmacal Research
1995 Volume.18 No. 1 p.12 ~ p.17
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Functional Experessions of Endogenous Dipeptide Transporter and Exogenous Proton/Peptide Cotransporter in Xenopus Oocytes
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Oh Doo-Man
Amidon-Gordon-L.
Sadee-Wolfgang
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Abstract
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It is essential to clone the preptide transporter in order to obtain better understanding of its molecular structure, regulation, and substrate specificity. Characteristics of an endogenous peptide transporter in oocytes were studied along with expression of an exogenous protor/peptide cotransporter from rabbit intestine. And further efforts toward cloning the transporter were performed. The presence of an endogenous peptide transporter was detected in Xenopus laevis oocytes by measuring the uptake of -glycylsarcosine (Gly-Sar) at pH 5.5 with or without inhibitors. Yptake of Gly-Sae in oocytes was significantly inhibited by glycine nd sarcosine. This result suggests that a selective transporter is involved in the endogneous uptake of dipeptides. Collagenase treatment of oocytes used to strip oocytes from ovarian follicles did not affect the Gly-Sar uptake. Changing pH from 5.5 to 7.5 did not affect the Gly-Sae uptake significantly, suggesting no depedence of the endogenous transporter on a transmembrane proton gradient. An exogenous contransported was expressed after microinjection of polyadenylated messenger ribonucleic acid obtained from rabbit small intestine. The Gly-Sar uptake in mRNA-injected oocytes was 9 times thigher than that in water-injected oocyltes. Thus, frog occytes can be utilized fro expression cloning of the genes encoding intestinal peptide contransporters. Size fractionation of mRNA was successfully obtained using this technique.
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KEYWORD
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Cotransporter, Dipeptide, Expression, Glycylsarcosine, Oocytes
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